Tumor Preparatio&
نویسنده
چکیده
The present work was conducted on Sarcoma 37 ascites tumor cells carried in CFW albino mice, Carworth Farms. The methods of inoculation, harvesting of cells, and prepa ration of homogenates were described previously (5). Glucose was determined by a specific Glucostat method (Worthington Biochemicals). Fructose was measured by the Anthrone method. Samples of hexose heated at 60°C permitted the determination of fructose in the presence of glucose. Samples heated to 100°Callowed both fructose and glucose to react and gave a colored product. Lactic acid was determined with the use of lactic dehydrogenase as the catalyst (Boehringer mid Soehne).3
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